3DBS-LNBioLiver Spheroid™

About

In Vitro MODEL 3DBS-LNBioLiverSpheroid™

In Vitro model developed by the Brazilian Biosciences National Laboratory – LNBio, part of the Brazilian Center for Research in Energy and Materials – CNPEM.

They are ideal for studies testing candidate drugs or biological products to treat diseases characterized by liver damage or absorption of substances in the liver. And also to analyze interactions between different models.

In Vitro model 3DBS-LNBioLiverSpheroid™

We develop alternative models of liver organoid to replace animal guinea pigs, providing the need for tests to evaluate the safety and efficacy of topical products such as medicines and cosmetics

Inspired by the structures of nature, our goal is to enable the reconstruction of tissues and biomimetic organs that act as native tissues

The model 3DBS-LNBioLiverSpheroid™ inserted in culture plates of 12 mm in diameter

CHARACTERIZATION OF IMAGE DIFFERENTIATION

Liver pethos iced optical microscopy with Hematoxylin & Eosin (H&E) after cuts of 5 one in paraffin in an increase of 20x (left) and characterization of liver beeferoid by confocal microscopy, presenting blue cell nucleus (DAPI) and endoplasmic reticulum in green (anti-CYP3A4), in increase and 20x (right).

CELL VIABILITY

Measurement of cell viability (%) of liver beeferoids within 21 days after biofabrication, demonstrating the high viability of the model.

GENE EXPRESSION

Expression of liver functionality markers on the first day of biofabrication until the 21 day, demonstrating an increase in the expression of markers characteristic of a real tissue.

Market segment:

Pharmaceutical and cosmetic industries: Research institutes, public and private universities

Use:

Search applications only

APPLICATIONS:

Use model 3DBS-LNBioLiverSpheroid to:

  • Toxicity: Potential to investigate the development, toxicity, as well as genetic and infectious diseases in ways currently limited by the availability of primary tissue.
  • Interactions: In contrast to current monolayer models, a differentiated beading can successfully mimic the multicellular complexity and 3D architecture that provides normal organ function, including cell-by-cell interaction.
  • Mimetizing native tissue: They can offer a potential advantage over non-human models, as they can examine the specific effects of the species in order to reduce or replace the animal models currently used, addressing important potential differences between species.
  • Drug Metabolism and Drug Delivery: predictive and human-relevant drug delivery tests to assess permeation, flow and flow transport, as well as metabolism.
  • Liver on Chip: The use of chip-based or microphysiological (MPS) culture systems offers many promising advantages in terms of environment control, reproducibility, feedback and automation when compared to traditional manual cell culture.